Don't play from a folder on the Desktop. Try closer to root, or a subfolder one away from \Program Files\. Not sure if this will work or not, but I've had issues with Desktop folders, and themes, resolutions, and DOSBOX. Has something to do with a program running ontop of a program, in different priorities, some memory sizes have real problems compensating for that.
Things closer to root directory, tend to execute off the HD faster. Thus keeping up with the RAM, which is prolly the fastest thing next to system processor and graphics processor.
Don't use DT as a guiding post. That app tends to be very tempermental, depending on the windows version, and cumulative multitasks. Sometimes my DT works like a charm, and then other times, it completely craps out. Not sure if its DT or Windows Defender, DEP(data execution protection) defending me from useless things. I can just picture DEP having issues with DT, even if I don't have it on good authority.